PNNL

Dr. Bohutskyi’s research focus is in developing new bioprocesses addressing sustainable transformation of carbon dioxide, biomass, and wet or liquid waste, including carbon, nitrogen, phosphorus, and other critical elements into bioproducts, chemicals, and fuels. This research uses a suite of microbial ecology, synthetic biology, multi-omic, and engineering approaches to gain insights into the biological processes and solve some of the world's biggest environmental, technological, and industrial challenges.

• Engineered and synthetic microbial communities
• Algal biotechnology
• Bioconversion of wastes into bioproducts, chemicals, and fuels
• Linking chemical and biological processes

• PhD in Environmental Engineering, Johns Hopkins University
• MS in Environmental Engineering, National Academy of Environmental Protection

2021

• Johnson Z.D., D. D. Krutkin, P. Bohutskyi, M. G. Kalyuzhnaya. 2021. “Metals and methylotrophy: Via global gene expression studies”. Methods in Enzymology. PNNL-SA-160052. doi:10.1016/bs.mie.2021.01.046

2020

• Frank B., D.G. Goodwin, P. Bohutskyi, D. Phan, X. Lu, L. Kuwama, and E. Bouwer, et al. 2020. "Influence of Polymer Type and Carbon Nanotube Properties on Carbon Nanotube/Polymer Nanocomposite Biodegradation." Science of the Total Environment 742. PNNL-SA-153893. doi:10.1016/j.scitotenv.2020.140512

2019

• Bohutskyi P., D. Phan, R. Spierling, A. Kopachevsky, E. Bouwer, T. Lundquist, and M. Betenbaugh. 2019. "Production of lipid-containing algal-bacterial polyculture in wastewater and biomethanation of lipid extracted residues: Enhancing methane yield through hydrothermal pretreatment and relieving solvent toxicity through co-digestion." Science of the Total Environment 653. PNNL-SA-137856. doi:10.1016/j.scitotenv.2018.11.026
• Bohutskyi P., R.S. McClure, E.A. Hill, W.C. Nelson, W.B. Chrisler, J. Nunez, and R.S. Renslow, et al. 2019. "Metabolic effects of vitamin B12 on physiology, stress resistance, growth rate and biomass productivity of Cyanobacterium stanieri planktonic and biofilm cultures." Algal Research 42. PNNL-SA-136164. doi:10.1016/j.algal.2019.101580
• Bohutskyi P., T.A. Keller, D. Phan, M.L. Parris, M. Li, L. Richardson, and A. Kopachevsky. 2019. "Co-digestion of Wastewater-Grown Filamentous Algae With Sewage Sludge Improves Biomethane Production and Energy Balance Compared to Thermal, Chemical, or Thermochemical Pretreatments." Frontiers in Energy Research 7. PNNL-SA-139910. doi:10.3389/fenrg.2019.00047

2018

• Bohutskyi P., D. Phan, A. Kopachevsky, S. Chow, E. Bouwer, and M. Betenbaugh. 2018. "Synergistic Co-Digestion of Wastewater Grown Algae-Bacteria Polyculture Biomass and Cellulose to Optimize Carbon-to-Nitrogen Ratio and Application of Kinetic Models to Predict Anaerobic Digestion Energy Balance." Bioresource Technology 269. PNNL-SA-134422. doi:10.1016/j.biortech.2018.08.085
• Bohutskyi P., L.A. Kucek, E.A. Hill, G.E. Pinchuk, S.G. Mundree, and A.S. Beliaev. 2018. "Conversion of stranded waste-stream carbon and nutrients into value-added products via metabolically coupled binary heterotroph-photoautotroph system." Bioresource Technology 260. PNNL-SA-126703. doi:10.1016/j.biortech.2018.02.080
• Bohutskyi P., R. Spierling, D. Phan, A. Kopachevsky, Y. Tang, M. Betenbaugh, and E. Bouwer, et al. 2018. "Bioenergy from wastewater resources: Nutrient removal, productivity and settleability of indigenous algal-bacteria polyculture, and effect of biomass composition variability on methane production kinetics and anaerobic digestion energy balance." Algal Research 36. PNNL-SA-134420. doi:10.1016/j.algal.2018.10.020
• Deng S., K.R. Pomraning, P. Bohutskyi, and J.K. Magnuson. 2018. "Draft Genome Sequence of Aspergillus oryzae ATCC 12892." Genome Announcements 8, no. 18:Article No. e00251-18. PNNL-SA-132840. doi:10.1128/genomeA.00251-18