Staff information

Research Interests

• nanotoxicology
• proteomics / protein biomarkers
• toxicity of metals
• computational toxicology

Education and Credentials

• B.A. Zoology, Chemistry, Olivet Nazarene College
• M.S. Environmental Toxicology, University of Wisconsin
• Ph.D. Environmental Toxicology, University of Wisconsin

Affiliations and Professional Service

• American Association for the Advancement of Science
• New York Academy of Sciences
• Scientific Research Society of North American (Sigma Xi)
• Society of Toxicology
• Member, External Advisory Committee, University of Arizona, Superfund Basic Research Program, 2004-present
• Member, Canada research Chairs Program College of Reviewers. 2002 - present
• Member, EPA Clean Air Science Advisory Committee, Lead Review Panel, 2010-present

Awards and Recognitions

• R&D 100 Award (1988) for development of X-Ray Microscope using Synchrotron Radiation
• Outstanding Young Men of America (1981)
• Commendable Service Award, Food and Drug Administration (1979) for meritorious research describing the percutaneous absorption of lead

PNNL Publications

2013

• Matzke MM, JN Brown, MA Gritsenko, TO Metz, JG Pounds, KD Rodland, AK Shukla, RD Smith, KM Waters, JE McDermott, and BJM Webb-Robertson. 2013. "A Comparative Analysis of Computational Approaches to Relative Protein Quantification Using Peptide Peak Intensities in Label-free LC-MS Proteomics Experiments." Proteomics 13(3-4):493-503.  doi:10.1002/pmic.201200269
• Tilton SC, KM Waters, NJ Karin, BJM Webb-Robertson, RC Zangar, MK Lee, DJ Bigelow, JG Pounds, and RA Corley. 2013. "Diet-Induced Obesity Reprograms the Inflammatory Response of the Murine Lung to Inhaled Endotoxin." Toxicology and Applied Pharmacology 262(2):137-148.  doi:10.1016/j.taap.2012.12.020
• Webb-Robertson BJM, MM Matzke, TO Metz, JE McDermott, J Walker, KD Rodland, JG Pounds, and KM Waters. 2013. "Sequential Projection Pursuit Principal Component Analysis - Dealing with Missing Data Associated with New -Omics Technologies." BioTechniques 54(3):165-168. 

2012

• Karakoti AS, P Munusamy, KE Hostetler, VK Kodali, SVNT Kuchibhatla, G Orr, JG Pounds, JG Teeguarden, BD Thrall, and DR Baer. 2012. "Preparation and Characterization Challenges to Understanding Environmental and Biological Impacts of Nanoparticles." Surface and Interface Analysis 44(8):881-889.  doi:10.1002/sia.5006
• Nian HC, J Wang, HJ Wu, JG Lo, KH Chiu, JG Pounds, and Y Lin. 2012. "Electrochemical Immunoassay of Cotinine in Serum Based on Nanoparticle Probe and Immunochromatographic Strip ." Analytica Chimica Acta 713:50-55.  doi:10.1016/j.aca.2011.11.028
• Varnum SM, BJM Webb-Robertson, JG Pounds, RJ Moore, RD Smith, C Frevert, SJ Skerret, and DS Wunschel. 2012. "Proteomic analysis of bronchoalveolar lavage fluid proteins from mice infected with Francisella tularensis ssp novicida." Journal of Proteome Research 11(7):3690-3703. 
• Xie Y, NG Williams, A Tolic, WB Chrisler, JG Teeguarden, BL Maddux, JG Pounds, A Laskin, and G Orr. 2012. "Aerosolized ZnO nanoparticles induce toxicity in alveolar type II epithelial cells at the air-liquid interface." Toxicological Sciences 125(2):450-461.  doi:10.1093/toxsci/kfr251

2011

• Jin H, BJM Webb-Robertson, ES Peterson, R Tan, DJ Bigelow, MB Scholand, JR Hoidal, JG Pounds, and RC Zangar. 2011. "Smoking, COPD and 3-Nitrotyrosine Levels of Plasma Proteins." Environmental Health Perspectives 119(9):1314-1320.  doi:10.1289/ehp.1103745
• Matzke MM, KM Waters, TO Metz, JM Jacobs, A Sims, R Baric, JG Pounds, and BJM Webb-Robertson. 2011. "Improved Quality Control Processing of Peptide-centric LC-MS Proteomics Data." Bioinformatics 27(20):2866-2872. 
• Teeguarden JG, BJM Webb-Robertson, KM Waters, A Murray, E Kisin, SM Varnum, JM Jacobs, JG Pounds, RC Zangar, and A Shvedova. 2011. "Comparative Proteomics and Pulmonary Toxicity of Instilled Single Walled Carbon Nanotubes, Crocidolite Asbestos and Ultrafine Carbon Black in Mice." Toxicological Sciences 120(1):123-135.  doi:10.1093/toxsci/kfq363
• Webb-Robertson BJM, MM Matzke, JM Jacobs, JG Pounds, and KM Waters. 2011. "A Statistical Selection Strategy for Normalization Procedures in LC-MS Proteomics Experiments through Dataset Dependent Ranking of Normalization Scaling Factors." Proteomics 11(24):4736-4741.  doi: 10.1002/pmic.201100078
• Zhang H, KE Burnum, ML Luna, BO Petritis, JS Kim, W Qian, RJ Moore, A Heredia-Langner, BJM Webb-Robertson, BD Thrall, DG Camp, II, RD Smith, JG Pounds, and T Liu. 2011. "Quantitative proteomics analysis of adsorbed plasma proteins classifies nanoparticles with different surface properties and size." Proteomics 11(23):4569-4577.  doi:10.1002/pmic.201100037

2010

• Hinderliter PM, KR Minard, G Orr, WB Chrisler, BD Thrall, JG Pounds, and JG Teeguarden. 2010. "ISDD: A Computational Model of Particle Sedimentation, Diffusion and Target Cell Dosimetry for In Vitro Toxicity Studies." Particle and Fibre Toxicology 7(November):Article No. 36.  doi:10.1186/1743-8977-7-36
• Li Z, Y Wang, J Wang, Z Tang, JG Pounds, and Y Lin. 2010. "Rapid and Sensitive Detection of Protein Biomarker Using a Portable Fluorescence Biosensor based on Quantum Dots and a Lateral Flow Test Strip." Analytical Chemistry 82(16):7008-7014.  doi:10.1021/ac101405a
• MCClay JL, DE Adkins, NG Isern, TM O'Connell, JB Wooten, BK Zedler, MS Dasika, BT Webb, BJM Webb-Robertson, JG Pounds, EL Murrelle, MF Leppert, and EJ van den Oord. 2010. "H-1 Nuclear Magnetic Resonance Metabolomics Analysis Identifies Novel Urinary Biomarkers for Lung Function." Journal of Proteome Research 9(6):3083-3090. 
• Rana GSJB, TP York, JS Edmiston, BK Zedler, JG Pounds, JN Adkins, RD Smith, Z Liu, G Li, BT Webb, EL Murrelle, and JW Flora. 2010. "Proteomic biomarkers in plasma that differentiate rapid and slow decline in lung function in adult cigarette smokers with chronic obstructive pulmonary disease (COPD) ." Analytical and Bioanalytical Chemistry 397:1809-1819.  doi:10.1007/s00216-010-3742-4
• Tang Z, H Wu, D Du, J Wang, H Wang, W Qian, DJ Bigelow, JG Pounds, RD Smith, and Y Lin. 2010. "Sensitive Immunoassays of Nitrated Fibrinogen in Human Biofluids." Talanta 81(4-5):1662-1669. 
• Webb-Robertson BJM, LA McCue, KM Waters, MM Matzke, JM Jacobs, TO Metz, SM Varnum, and JG Pounds. 2010. "Combined Statistical Analyses of Peptide Intensities and Peptide Occurrences Improves Identification of Significant Peptides from MS-based Proteomics Data." Journal of Proteome Research 9(11):5748-5756. 
• Zhang X, ME Monroe, B Chen, MH Chin, TH Heibeck, AA Schepmoes, F Yang, BO Petritis, DG Camp, II, JG Pounds, JM Jacobs, DJ Smith, DJ Bigelow, RD Smith, and W Qian. 2010. "Endogenous 3, 4- Dihydroxyphenylalanine and Dopaquinone Modifications on Protein Tyrosine: links to mitochondrially derived oxidative stress via hydroxyl radical." Molecular & Cellular Proteomics. MCP 9(6):1199-1208. 

2009

• Orr G, DJ Panther, KJ Cassens, JL Phillips, BJ Tarasevich, and JG Pounds. 2009. "Syndecan-1 Mediates the Coupling of Positively Charged Submicrometer Amorphous Silica Particles with Actin Filaments across the Alveolar Epithelial Cell Membrane." Toxicology and Applied Pharmacology 236(2):210-220.  doi:10.1016/j.taap.2009.01.022
• Waters KM, LM Masiello, RC Zangar, BJ Tarasevich, NJ Karin, RD Quesenberry, S Bandyopadhyay, JG Teeguarden, JG Pounds, and BD Thrall. 2009. "Macrophage Responses to Silica Nanoparticles are Highly Conserved Across Particle Sizes." Toxicological Sciences 107(2):553-569. 
• Webb-Robertson BJM, LA McCue, N Beagley, JE McDermott, DS Wunschel, SM Varnum, JZ Hu, NG Isern, GW Buchko, K Mcateer, JG Pounds, SJ Skerret, D Liggitt, and CW Frevert. 2009. "A Bayesian Integration Model of High-Throughput Proteomics and Metabolomics Data for Improved Early Detection of Microbial Infections." In Pacific Symposium on Biocomputing, vol. 14, pp. 451-463.  World Scientific Publishing Co., Singapore, Singapore. 

2008

• Pounds JG, JW Flora, JN Adkins, KM Lee, GSJB Rana, T Sengupta, RD Smith, and WJ McKinney. 2008. "Characterization of the mouse bronchoalveolar lavage proteome by micro-capillary LC-FTICR mass spectrometry." Journal of Chromatography B 864(1-2):95-101. 
• Whittmers Jr. LE, AC Aufderheide, JG Pounds, K JONES, and JL Angel. 2008. "Problems in Determination of Skeletal Lead Burden in Archaeological Samples: An Example From the First African Baptist Church Population ." American Journal of Physical Anthropology 136(4):379-386. 

2007

• Orr G, DJ Panther, JL Phillips, BJ Tarasevich, A Dohnalkova, D Hu, JG Teeguarden, and JG Pounds. 2007. "Submicron and Nanoscale Inorganic Particles Exploit the Actin Machinery to be Propelled Along Microvillilike Structures into Alveolar Cells." ACS Nano 1(5):463-475.  doi:10.1021/nn700149r
• Teeguarden JG, PM Hinderliter, G Orr, BD Thrall, and JG Pounds. 2007. "Particokinetics In Vitro: Dosimetry Considerations for In Vitro Nanoparticle Toxicity Assessments." Toxicological Sciences 95(2):300-312.  doi:10.1093/toxsci/kfl165

2006

• Coon S, A Stark, E Peterson, A Gloi, G Kortsha, JG Pounds, DR Chettle, and JM Gorell. 2006. "Whole-Body Lifetime Occupational Lead Exposure and Risk of Parkinson's Disease." Environmental Health Perspectives 114(12):1872-6. 
• Stork LAM, C Gennings, R Carchman, WH Carter, Jr., JG Pounds, and M Mumtaz. 2006. "Testing for Additivity at Select Mixture Groups of Interest Based on Statistical Equivalence Testing Methods." Risk Analysis 26(6):1601-1612. 
• Waters KM, JG Pounds, and BD Thrall. 2006. "Data Merging for Integrated Microarray and Proteomic Analysis." Briefings in Functional Genomics and Proteomics 5(4):261-272.  doi:10.1093/bfgp/ell019

2005

• Adkins JN, ME Monroe, KJ Auberry, Y Shen, JM Jacobs, DG Camp, II, F Vitzthum, KD Rodland, RC Zangar, RD Smith, and JG Pounds. 2005. "A Proteomic Study of the HUPO Plasma Proteome Project's Pilot Samples using an Accurate Mass and Time Tag Strategy." Proteomics 5(13):3454-3466.  doi:10.1002/pmic.200401333
• Omenn G, DJ States, M Adamski, TW Blackwell, R Menon, H Hermjakob, R Apweiler, BB Haab, R Simpson, J Eddes, E Kapp, R Moritz, DW Chan, AJ Rai, A Admon, R Aebersold, JK Eng, WS Hancock, SA Hefta, H Meyer, YK Paik, JS Yoo, P Ping, JG Pounds, JN Adkins, X Qian, R Wang, V Wasinger, CY Wu, X Zhao, R Zeng, A Archakov, A Tsugita, I Beer, A Pandey, M Pisano, P Andrews, H Tammen, DW Speicher, and SM Hanash. 2005. "Overview of the HUPO Plasma Proteome Project: Results from the pilot phase with 35 collaborating laboratories and multiple analytical groups, generating a core dataset of 3020 proteins and a publicly-available database ." Proteomics 5(13):3226-3245. 
• Webb-Robertson BJM, DF Lowry, KH Jarman, SJ Harbo, Q Meng, AF Fuciarelli, JG Pounds, and MT Lee. 2005. "A Study of Spectral Integration and Normalization in NMR-based Metabonomic Analyses ." Journal of Pharmaceutical and Biomedical Analysis 39(3-4):830-836. 

2004

• Anderson LN, M Polanski, R Peiper, T Gatlin, R Tiramalai, T Conrads, T Veenstra, JN Adkins, JG Pounds, R Fagan, and A Lobley. 2004. "The Human Plasma Proteome: A Nonredundant List Developed by Combination of Four Separate Sources ." Molecular & Cellular Proteomics. MCP 3(4):311-326.  doi:10.1074/mcp.M300127-MCP200

2003

• Varnum SM, CC Covington, RL Woodbury, K Petritis, LJ Kangas, MS Abdullah, JG Pounds, RD Smith, and RC Zangar. 2003. "Proteomic Characterization of Nipple Aspirate Fluid: Identification of Potential Biomarkers of Breast Cancer ." Breast Cancer Research and Treatment 80(1):87-97. 
• Varnum SM, CC Covington, RL Woodbury, K Petritis, LJ Kangas, MS Abdullah, JG Pounds, RD Smith, and RC Zangar. 2003. "Proteomic Characterization of Nipple Aspirate Fluid: Identification of Potential Biomarkers of Breast Cancer." Breast Cancer Research and Treatment 80(1):87-97. 
• Varnum SM, CC Covington, RL Woodbury, K Petritis, LJ Kangas, MS Abdullah, JG Pounds, RD Smith, and RC Zangar. 2003. "Proteomic characterization of nipple aspirate fluid: Identification of potential biomarkers of breast cancer." Breast Cancer Research and Treatment 80(1):87-97.  doi:10.1023/A:1024479106887

2002

• Adkins JN, SM Varnum, KJ Auberry, RJ Moore, N Angell, RD Smith, DL Springer, and JG Pounds. 2002. "Toward a Human Blood Serum Proteome: Analysis by Multidimensional Separation Coupled with Mass Spectrometry." Molecular & Cellular Proteomics. MCP 1(12):947-955. 

2000

• Mccabe MJ, KP Singh, SA Reddy, BS Chelladurai, JG Pounds, JJ Reiners, and JC States. 2000. "Sensitivity of Myetomonocytic Leukemia Cells to Arsenite-Induced Cell Cycle Disruption, Apoptosis and Enhanced Differentiation is Dependent on the Interrelationship Between Arsenic Concentration, Duration of Treatment and Cell Cycle Phase." Journal of pharmacology and experimental therapeutics 295(2):724-733.