ABSTRACT: Total DNA from sediment samples was isolated by a direct lysis technique. Purified DNA was used as template either undiluted or diluted 1:10 prior to polymerase chain reaction (PCR) amplificatio of 16S rRNA genes.
Revised: July 11, 2019 |
Published: May 1, 1997
Citation
Chandler D.P., J.K. Fredrickson, and F.J. Brockman. 1997.Effect of PCR template concentration on the composition and distribution of total community 16S rDNA clone libraries.Molecular Ecology 6, no. 5:475-482.PNNL-SA-27281.doi:10.1046/j.1365-294X.1997.00205.x