Biomedical Scientist
Biomedical Scientist

Biography

Josh Hansen is a biomedical scientist in the Biological Sciences Division. His research focuses on processing clinical samples and expanding development of the microPOTS pipeline for the biosample pipeline team.

Hansen contributes to many collaborator papers and projects related to the biosample pipeline. His main pipeline follows the Clinical Proteomic Tumor Analysis Consortium’s protocol, and he’s now applying the microPOTS protocol to small scale samples.

Other areas of interest include genetics, regenerative medicine, and automation.

Disciplines and Skills

  • Bicinchoninic acid protein assay
  • Biohazard
  • Biology
  • Biomarker discovery
  • Biomedical sciences
  • Biosafety
  • Clinical research
  • Peptides
  • Protein purification
  • Protocol development
  • Sample management
  • Sample preparation
  • Teamwork

Education

  • MS in Molecular Biosciences, Washington State University
  • BS in General Biology, Washington State University

Publications

2020

  • J.A. Sanford, C.D. Nogiec, M.E. Lindholm, J.N. Adkins, D. Amar, S. Dasari, J.K. Drugan, F.M. Fernandez, S. Radom-Aizik, S. Schenk, M.P. Snyder, R.P. Tracy, P. Vanderboom, S. Trappe, M.J. Walsh, Molecular Transducers of Physical Activity Consortium. “Molecular Transducers of Physical Activity Consortium (MoTrPAC): Mapping the Dynamic Responses to Exercise.” Cell 181, No 7:1464-1474. doi:10.1016/j.cell.2020.06.004
  • J.A. Sanford, Y. Wang, J.R. Hansen, M.A. Gritsenko, K.K. Weitz, C.E. Tognon, V.A. Petyuk, et al. “Evaluation of differential peptide loading on tandem mass tag-based proteomic and phosphoproteomic data quality.” Clinical Proteomics. doi:10.21203/rs.3.rs-51123/v1. In review.
  • T.K. Sigdel, P.D. Piehowski, S. Roy, J. Liberto, J.R. Hansen, A.C. Swensen, J. Luo, Y. Yang, P. Rashmi, A. Schroeder, I. Damm, S. Sur, W. Qian, M. Sarwal. “Near-Single Cell Proteomics Profiling of the Proximal Tubular and Glomerulus of the Normal Human Kidney.” Front. Med. Nephrology.Doi:10.3389/fmed.2020.00499

2019

  • Dickinson M., L.N. Anderson, B.M. Webb-Robertson, J.R. Hansen, R.D. Smith, A.T. Wright, and K. Hybiske. 2019. "Proximity-dependent proteomics of the Chlamydia trachomatis inclusion membrane reveals functional interactions with endoplasmic reticulum exit sites." PLoS Pathogens 15, no. 4:Article Number e1007698. PNNL-SA-132851. doi:10.1371/journal.ppat.1007698
  • Whidbey C., N.C. Sadler, R.N. Nair, R.F. Volk, A.J. DeLeon, L.M. Bramer, and S.J. Fansler, et al. 2019. "A Probe-Enabled Approach for the Selective Isolation and Characterization of Functionally Active Subpopulations in the Gut Microbiome." Journal of the American Chemical Society 141, no. 1:42-47. PNNL-SA-127532. doi:10.1021/jacs.8b09668

2018

  • Ortega C., A. Frando, B.M. Webb-Robertson, L.N. Anderson, N. Fleck, E.L. Flannery, and M. Fishbaugher, et al. 2018. "A global survey of ATPase activity in Plasmodium falciparum asexual blood stages and gametocytes." Molecular and Cellular Proteomics 17, no. 1:111-120. PNNL-SA-121236. doi:10.1074/mcp.RA117.000088

2017

  • Smith J.N., K.J. Tyrrell, J.R. Hansen, D.G. Thomas, T.A. Murphree, A.K. Shukla, and T. Luders, et al. 2017. "Plasma Protein Turnover Rates in Rats Using Stable Isotope Labeling, Global Proteomics, and Activity-Based Protein Profiling." Analytical Chemistry 89, no. 24:13559-13566. PNNL-SA-129561. doi:10.1021/acs.analchem.7b03984