High-Throughput Proteomics for Trauma Research with the Inflammation and the Host Response to Injury Consortium
Sponsor: National Institutes of Health
Contact: David Camp
The host response to trauma and burns is a highly complex biological and pathological process that depends critically upon the regulation of the human immuno-inflammatory response. This large-scale collaborative project is applying high-throughput biological discovery tools to identify important dynamic relationships that regulate this response, with the expectation that this knowledge will ultimately impact the treatment of burn and trauma patients. We are developing an LC-FTICR approach using yeast as a model system and testing this approach by measuring a battery of cytokines in the serum of trauma patients. We also are investigating transient post-translational protein modifications in relevant proteins to provide additional information on critical participants in the signal transduction pathways influenced by cytokine binding.