Biomarkers
Scientists at PNNL are focused on identification of unique and integrated biomarkers of exposure using multiplexed platforms, including proteomics, and antibody microarrays. For example, microarray ELISA technology developed at PNNL was recently used to conduct measures of oxidative protein modifications during lung disease progression in humans (24 ELISAs on 458 individuals, or 32,976 ELISAs total).
Diagram of a single sandwich ELISA. The capture antibody (Ab) concentrates the antigen (Ag) onto the surface of the glass slide. A second antibody that also binds the same antigen then detects the amount of the antigen captured by the first antibody.
Capabilities
We developed a multiplex sandwich ELISA microarray platform that allows for the rapid, high-throughput, quantitative analysis of multiple, low-abundance proteins in hundreds of samples. We have applied this platform to the analysis of intact and modified proteins.
Expertise
Biomarkers of disease, including oxidative protein modifications.
Current projects
- National Cancer Institute: Validation of Plasma Biomarkers of Triple Negative Breast Cancer
- National Institute of Environmental Health: P50 Center for Biomarkers of Response.
- PNNL Laboratory Directed Research and Development: Capabilities for Analyzing Post-translational Protein Modifications.
Investigators: Clockwise, from left, Elena Peterson, Rick Zangar, Ruimin Tan, Joel Pounds, Diana Bigelow, Bobbie-Jo Webb-Robertson
Selected Publications:
Zangar RC, N Bollinger, TJ Weber, R Tan, LM Markillie, and NJ Karin. 2011. "Reactive Oxygen Species Alter Autocrine and Paracrine Signaling." Free Radical Biology & Medicine 51(11):2041-2047. DOI: 10.1016/j.freeradbiomed.2011.09.001
Jin H, B-J Webb-Robertson, ES Peterson, R Tan, DJ Bigelow, MB Scholand, JR Hoidal, JG Pounds, and RC Zangar. 2011. "Smoking, COPD, and 3-Nitrotyrosine Levels of Plasma Proteins." Environmental Health Perspectives 119(9):1314-1320. DOI: 10.1289/ehp.1103745
Gonzales RM, DS Daly, R Tan, JR Marks, and RC Zangar. 2011. "Plasma Biomarker Profiles Differ Depending on Breast Cancer Subtype but RANTES is Consistently Increased." Cancer Epidemiology, Biomarkers and Prevention 20(7):1543-1551. DOI: 10.1158/1055-9965.EPI-10-1248
Daly DS, KK Anderson, SL Seurynck-Servoss, RM Gonzalez, AM White, and RC Zangar. 2010. "An Internal Calibration Method for Protein-Array Studies." Statistical Applications in Genetics and Molecular Biology 9(1):Article Number: 14. DOI: 10.2202/1544-6115.1506
Gonzalez RM, S Seurynck-Servoss, SA Crowley, M Brown, G Omenn, D Hayes, and RC Zangar. 2008. "Development and Validation of Sandwich ELISA Microarrays with Minimal Assay Interference." Journal of Proteome Research 7, 2406-2414. DOI: 10.1021/pr700822t
Seurynck-Servoss SL, CL Baird, KD Rodland, and RC Zangar. 2007. "Surface Chemistries for Antibody Microarrays." Frontiers in Bioscience 12:3956-3964. DOI: 10.2741/2362
White AM, DS Daly, SM Varnum, KK Anderson, N Bollinger, and RC Zangar. 2006. "ProMAT: Protein Microarray Analysis Tool." Bioinformatics 22(10):1278-1279. DOI: 10.1093/bioinformatics/btl093
Research Highlights
Addressing How Cigarettes Cause Cardiovascular Disease Addressing High False-Positive Rates for Mammograms NIH FundsNew Biomarkers Center at PNNL Open-source Software Tool for Protein Microarray Analysis Available from PNNL